1. Mateos, P., Velázquez, E., Tejedor, C., Monte, E., Vizcaíno, N., and Jiménez-Zurdo, J.I. 1991. MacMic: A computer program for teaching microbial systematics by simulation. Binary. 3: 179-182.
2. Fernández-Lago, L., Santoyo, F.J., Vizcaíno, N., and Chordi, A. 1991. Class-specific immune response to Yersinia enterocolitica serotype O9 antigens as determined by enzyme-linked immunosorbent assay. J. Clin. Microbiol. 29(6): 1243-1248.
3. Vizcaíno, N., Chordi, A., and Fernández-Lago, L. 1991. Characterization of smooth Brucella lipopolysaccharides and polysaccharides by monoclonal antibodies. Res Microbiol. 142(9): 971-978.
4. Vizcaíno, N. and Fernández-Lago, L. 1992. A rapid and sensitive method for the identification of Brucella species with a monoclonal antibody. Res. Microbiol. 143(5): 513-518.
5. Vizcaíno, N. and Fernández-Lago, L. 1994. Protection and suppression of the humoral immune response in mice mediated by a monoclonal antibody against the M epitope of Brucella. FEMS Immunol. Med. Microbiol. 8(2): 133-139.
6. Fernández-Lago, L., Gómez, M., Vizcaíno, N., and Chordi, A. 1994. Analysis of the immune response to Yersinia enterocolitica serotype-O:9-released proteins by immunoblot and ELISA. Res. Microbiol. 145(7): 553-561.
7. Fernández-Lago, L., Gómez, M., Vizcaíno, N., and Chordi, A. 1995. Immunological study of the expression of proteins encoded by the virulence plasmid of Yersinia enterocolitica serotype O:9. Contrib. Microbiol. Immunol. 13: 256-261.
8. Cloeckaert, A., Debbarh, H.S., Vizcaíno, N., Saman, E., Dubray, G., and Zygmunt, M.S. 1996. Cloning, nucleotide sequence, and expression of the Brucella melitensis bp26 gene coding for a protein immunogenic in infected sheep. FEMS Microbiol. Lett. 140(2-3): 139-144.
9. Vizcaíno, N., Cloeckaert, A., Dubray, G., and Zygmunt, M.S. 1996. Cloning, nucleotide sequence, and expression of the gene coding for a ribosome releasing factor-homologous protein of Brucella melitensis. Infect. Immun. 64(11): 4834-4837.
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11. Vizcaíno, N., Cloeckaert, A., Zygmunt, M.S., and Dubray, G. 1996. Cloning, nucleotide sequence, and expression of the Brucella melitensis omp31 gene coding for an immunogenic major outer membrane protein. Infect. Immun. 64(9): 3744-3751.
12. Vizcaíno, N., Verger, J.M., Grayon, M., Zygmunt, M.S., and Cloeckaert, A. 1997. DNA polymorphism at the omp-31 locus of Brucella spp.: evidence for a large deletion in Brucella abortus, and other species-specific markers. Microbiology. 143 ( Pt 9): 2913-2921.
13. Vizcaíno, N., Zygmunt, M.S., Verger, J.M., Grayon, M., and Cloeckaert, A. 1997. Localization and characterization of a specific linear epitope of the Brucella DnaK protein. FEMS Microbiol. Lett. 154(1): 117-122.
14. Kittelberger, R., Diack, D.S., Vizcaíno, N., Zygmunt, M.S., and Cloeckaert, A. 1998. Characterization of an immuno-dominant antigen in Brucella ovis and evaluation of its use in an enzyme-linked immunosorbent assay. Vet Microbiol. 59(2-3): 213-227.
15. Reyna-Bello, A., Cloeckaert, A., Vizcaíno, N., Gonzatti, M.I., Aso, P.M., Dubray, G., and Zygmunt, M.S. 1998. Evaluation of an enzyme-linked immunosorbent assay using recombinant major surface protein 5 for serological diagnosis of bovine anaplasmosis in Venezuela. Clin. Diagn. Lab. Immunol. 5(2): 259-262.
16. Fernández-Lago, L., Rodríguez-Tarazona, E., and Vizcaíno, N. 1999. Differential secretion of interleukin-12 (IL-12) subunits and heterodimeric IL-12p70 protein by CD-1 mice and murine macrophages in response to intracellular infection by Brucella abortus. J. Med. Microbiol. 48(12): 1065-1073.
17. Guilloteau, L.A., Laroucau, K., Vizcaíno, N., Jacques, I., and Dubray, G. 1999. Immunogenicity of recombinant Escherichia coli expressing the omp31 gene of Brucella melitensis in BALB/c mice. Vaccine. 17(4): 353-361.
18. Vizcaíno, N., Cloeckaert, A., Zygmunt, M.S., and Fernandez-Lago, L. 1999. Molecular characterization of a Brucella species large DNA fragment deleted in Brucella abortus strains: evidence for a locus involved in the synthesis of a polysaccharide. Infect. Immun. 67(6): 2700-2712.
19. Fernández-Lago, L., Vallejo, F.J., Trujillano, I., and Vizcaíno, N. 2000. Fluorescent whole-cell hybridization with 16S rRNA-targeted oligonucleotide probes to identify Brucella spp. by flow cytometry. J. Clin. Microbiol. 38(7): 2768-2771.
20. Vizcaíno, N., Cloeckaert, A., Verger, J., Grayon, M., and Fernández-Lago, L. 2000. DNA polymorphism in the genus Brucella. Microbes Infect. 2(9): 1089-1100.
21. Cloeckaert, A., Baucheron, S., Vizcaíno, N., and Zygmunt, M.S. 2001. Use of recombinant BP26 protein in serological diagnosis of Brucella melitensis infection in sheep. Clin. Diagn. Lab. Immunol. 8(4): 772-775.
22. Vizcaíno, N., Cloeckaert, A., Zygmunt, M.S., and Fernández-Lago, L. 2001. Characterization of a Brucella species 25-kilobase DNA fragment deleted from Brucella abortus reveals a large gene cluster related to the synthesis of a polysaccharide. Infect Immun. 69(11): 6738-6748.
23. Vizcaíno, N., Kittelberger, R., Cloeckaert, A., Marín, C.M., and Fernández-Lago, L. 2001. Minor nucleotide substitutions in the omp31 gene of Brucella ovis result in antigenic differences in the major outer membrane protein that it encodes compared to those of the other Brucella species. Infect. Immun. 69(11): 7020-7028.
24. Rivas, R., Vizcaíno, N., Buey, R.M., Mateos, P.F., Martínez-Molina, E., and Velázquez, E. 2001. An effective, rapid and simple method for total RNA extraction from bacteria and yeast. J. Microbiol. Methods. 47(1): 59-63.
25. Cloeckaert, A., Vizcaíno, N., Paquet, J.Y., Bowden, R.A., and Elzer, P.H. 2002. Major outer membrane proteins of Brucella spp.: past, present and future. Vet. Microbiol. 90(1-4): 229-247.
26. Zygmunt, M.S., Baucheron, S., Vizcaíno, N., Bowden, R.A., and Cloeckaert, A. 2002. Single-step purification and evaluation of recombinant BP26 protein for serological diagnosis of Brucella ovis infection in rams. Vet. Microbiol. 87(3): 213-220.
27. Rivas, R., Velazquez, E., Willems, A., Vizcaíno, N., Subba-Rao, N.S., Mateos, P.F., Gillis, M., Dazzo, F.B., and Martínez-Molina, E. 2002. A new species of Devosia that forms a unique nitrogen-fixing root-nodule symbiosis with the aquatic legume Neptunia natans (L.f.) druce. Appl. Environ. Microbiol. 68(11): 5217-5222.
28. Estein, S.M., Cassataro, J., Vizcaíno, N., Zygmunt, M.S., Cloeckaert, A., and Bowden, R.A. 2003. The recombinant Omp31 from Brucella melitensis alone or associated with rough lipopolysaccharide induces protection against Brucella ovis infection in BALB/c mice. Microbes Infect. 5(2): 85-93.
29. Seco-Mediavilla, P., Verger, J.M., Grayon, M., Cloeckaert, A., Marin, C.M., Zygmunt, M.S., Fernández-Lago, L., and Vizcaíno, N. 2003. Epitope mapping of the Brucella melitensis BP26 immunogenic protein: usefulness for diagnosis of sheep brucellosis. Clin. Diagn. Lab. Immunol. 10(4): 647-651.
30. Vizcaíno, N., Caro-Hernández, P., Cloeckaert, A., and Fernández-Lago, L. 2004. DNA polymorphism in the omp25/omp31 family of Brucella spp.: identification of a 1.7-kb inversion in Brucella cetaceae and of a 15.1-kb genomic island, absent from Brucella ovis, related to the synthesis of smooth lipopolysaccharide. Microbes Infect. 6(9): 821-834.
31. Fernández-Lago, L., Orduña, A., and Vizcaíno, N. 2005. Reduced interleukin-18 secretion in Brucella abortus 2308-infected murine peritoneal macrophages and in spleen cells obtained from B. abortus 2308-infected mice. J. Med. Microbiol. 54(Pt 6): 527-531.
32. Valverde, A., Velazquez, E., Fernández-Santos, F., Vizcaíno, N., Rivas, R., Mateos, P.F., Martínez-Molina, E., Igual, J.M., and Willems, A. 2005. Phyllobacterium trifolii sp. nov., nodulating Trifolium and Lupinus in Spanish soils. Int. J. Syst. Evol. Microbiol. 55(Pt 5): 1985-1989.
33. Mantecón, M.A., Gutiérrez, P., Zarzosa, M.P., Dueñas, A.I., Solera, J., Fernández-Lago, L., Vizcaíno, N., Almaraz, A., Bratos, M.A., Rodríguez-Torres, A., and Orduña-Domingo, A. 2006. Utility of an immunocapture-agglutination test and an enzyme-linked immunosorbent assay test against cytosolic proteins from Brucella melitensis B115 in the diagnosis and follow-up of human acute brucellosis. Diagn Microbiol Infect Dis. 55(1): 27-35.
34. Zurdo-Piñeiro, J.L., Rivas, R., Trujillo, M.E., Vizcaíno, N., Carrasco, J.A., Chamber, M., Palomares, A., Mateos, P.F., Martínez-Molina, E., and Velázquez, E. 2007. Ochrobactrum cytisi sp. nov. isolated from nodules of Cytisus scoparius in Spain. Int J Syst Evol Microbiol. 57(Pt 4): 784-788.
35. Caro-Hernández, P., Fernández-Lago, L., de Miguel, M.J., Martín-Martín, A.I., Cloeckaert, A., Grilló, M.J., and Vizcaíno, N. 2007. Role of the Omp25/Omp31 family in outer membrane properties and virulence of Brucella ovis. Infect. Immun. 75(8): 4050-4061.
36. Jacques, I., Verger, J.M., Laroucau, K., Grayon, M., Vizcaíno, N., Peix, A., Cortade, F., Carreras, F., and Guilloteau, L.A. 2007. Immunological responses and protective efficacy against Brucella melitensis induced by bp26 and omp31 B. melitensis Rev.1 deletion mutants in sheep. Vaccine. 25(5): 794-805.
37. Martín-Martín, A.I., Caro-Hernández, P., Orduña, A., Vizcaíno, N., and Fernández-Lago, L. 2008. Importance of the Omp25/Omp31 family in the internalization and intracellular replication of virulent B. ovis in murine macrophages and HeLa cells. Microbes Infect. 10(6): 706-710.
38. Mantecón, M.A., Gutiérrez, M.P., Zarzosa, M.P., Fernández-Lago, L., Colmenero, J.D., Vizcaíno, N., Bratos, M.A., Almaraz, A., Cubero, A., Muñoz, M.F., Rodríguez Torres, A., and Orduña, A. 2008. Influence of brucellosis history on serological diagnosis and evolution of patients with acute brucellosis. J Infect. 57(5): 397-403.
39. Martín-Martín, A.I., Caro-Hernández, P., Sancho, P., Tejedor, C., Cloeckaert, A., Fernández-Lago, L., and Vizcaíno, N. 2009. Analysis of the occurrence and distribution of the Omp25/Omp31 family of surface proteins in the six classical Brucella species. Vet Microbiol. 137: 74-82.
40. Martín-Martín, A.I., Vizcaíno, N., and Fernández-Lago, L. 2010. Cholesterol, ganglioside GM1 and class A scavenger receptor contribute to infection by Brucella ovis and Brucella canis in murine macrophages. Microbes Infect. 12(3): 246-251.
41. Martín-Martín, A.I., Sancho, P., Tejedor, C., Fernández-Lago, L., and Vizcaíno, N. 2011. Differences in the outer membrane-related properties of the six classical Brucella species. Vet. J. 189(1): 103-105.
42. Martín-Martín, A.I., Sancho, P., de Miguel, M.J., Fernández-Lago, L., and Vizcaíno, N. 2012. Quorum-sensing and BvrR/BvrS regulation, the type IV secretion system, cyclic glucans, and BacA in the virulence of Brucella ovis: similarities to and differences from smooth brucellae. Infect. Immun. 80(5): 1783-1793.
43. Sancho, P., Tejedor, C., Sidhu-Muñoz, R.S., Fernández-Lago, L., and Vizcaíno, N. 2014. Evaluation in mice of Brucella ovis attenuated mutants for use as live vaccines against B. ovis infection. Vet. Res. 45: 61. doi:10.1186/1297-9716-45-61
44. Soler-Lloréns, P., Gil-Ramírez, Y., Zabalza-Baranguá, A., Iriarte, M., Conde-Álvarez, R., Zúñiga-Ripa, A., San Román, B., Zygmunt, M.S., Vizcaíno, N., Cloeckaert, A., Grilló, M.J., Moriyón, I., and López-Goñi, I. 2014. Mutants in the lipopolysaccharide of Brucella ovis are attenuated and protect against B. ovis infection in mice. Vet. Res. 45(1): 72. doi:10.1186/s13567-014-0072-0
45. Sidhu-Muñoz, R.S., Sancho, P., and Vizcaíno, N. 2016. Brucella ovis PA mutants for outer membrane proteins Omp10, Omp19, SP41, and BepC are not altered in their virulence and outer membrane properties. Vet. Microbiol. 186: 59-66.
46. Sidhu-Muñoz, R.S., Sancho, P., Cloeckaert, A., Zygmunt, M.S., de Miguel, M.J., Tejedor, C., and Vizcaíno, N. 2018. Characterization of cell envelope multiple mutants of Brucella ovis and assessment in mice of their vaccine potential. Front. Microbiol. 9(2230). doi: 10.3389/fmicb.2018.02230
47. Sidhu-Muñoz, R.S., Sancho, P., and Vizcaíno, N. 2018. Evaluation of human trophoblasts and ovine testis cell lines for the study of the intracellular pathogen Brucella ovis. FEMS Microbiol Lett. 365(24). doi:10.1093/femsle/fny278
48. Sidhu-Muñoz, R.S., Tejedor, T., and Vizcaíno, N. 2020. The three flagellar loci of Brucella ovis PA are dispensable for virulence in cellular models and mice. Front. Vet. Sci. 7:441. doi: 10.3389/fvets.2020.00441
49. Vizcaíno, N., Pérez-Etayo, L., Conde-Álvarez, R., Iriarte, M., Moriyón, I., and Zúñiga-Ripa, A., Disruption of pyruvate phosphate dikinase in Brucella ovis PA CO2-dependent and independent strains generates attenuation in the mouse model. Vet Res, 2020. 51(1): 101. doi:10.1186/s13567-020-00824-7
50. Cloeckaert, A., Zygmunt, M.S., Scholz, H.C., Vizcaino, N., and Whatmore, A.M., Editorial: Pathogenomics of the Genus Brucella and Beyond. Front Microbiol, 2021. 12: 700734. doi:10.3389/fmicb.2021.700734
